A little guide for advanced search:

Tip 1. You can use quotes "" to search for an exact expression.
Example: "cell division"
Tip 2. You can use + symbol to restrict results containing all words.
Example: +cell +stem
Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
Example: +cell -stem

e.g. searching for members in projects tagged cancer

Search for

Count

IN

OUT

Content 1

Content Type

member
team
department
center
program_project
nrc
whocc
project
software
tool
patent

Keywords

Positions

Administrative Staff
Assistant Professor
Associate Professor
Clinical Research Assistant
Clinical Research Nurse
Clinician Researcher
Department Manager
Dual-education Student
Full Professor
Honorary Professor
Lab assistant
Master Student
Non-permanent Researcher
Nursing Staff
Permanent Researcher
Pharmacist
PhD Student
Physician
Post-doc
Prize
Project Manager
Research Associate
Research Engineer
Retired scientist
Technician
Undergraduate Student
Veterinary
Visiting Scientist

Appointments

Deputy Director of Center
Deputy Director of Department
Deputy Director of National Reference Center
Deputy Head of Facility
Director of Center
Director of Department
Director of Institute
Director of National Reference Center
Group Leader
Head of Facility
Head of Operations
Head of Structure
Honorary President of the Departement
Labex Coordinator

Content 2

Content Type

member
team
department
center
program_project
nrc
whocc
project
software
tool
patent

Keywords

Positions

Administrative Staff
Assistant Professor
Associate Professor
Clinical Research Assistant
Clinical Research Nurse
Clinician Researcher
Department Manager
Dual-education Student
Full Professor
Honorary Professor
Lab assistant
Master Student
Non-permanent Researcher
Nursing Staff
Permanent Researcher
Pharmacist
PhD Student
Physician
Post-doc
Prize
Project Manager
Research Associate
Research Engineer
Retired scientist
Technician
Undergraduate Student
Veterinary
Visiting Scientist

Appointments

Deputy Director of Center
Deputy Director of Department
Deputy Director of National Reference Center
Deputy Head of Facility
Director of Center
Director of Department
Director of Institute
Director of National Reference Center
Group Leader
Head of Facility
Head of Operations
Head of Structure
Honorary President of the Departement
Labex Coordinator

Search

EN
FR

Go back

Scroll to top

Journée de la recherche translationnelle – Vaccine & HBV/HIV Cure

BCI Seminar – “Autophagy and Neurodegeneration”

© William Beaucardet

Reportage Laboratoire des Anticorps en Thérapie et Pathologie

Event

MicroOmiX : Enabling droplet microfluidics technologies

Team: UTechS Single Cell Biomarkers

Center: Center for Technological Resources and Research

Member: Pierre-Henri Commere

Member: Mariette Matondo

Member: Sophie Novault

Scientific Fields

Diseases

Organisms

Applications

Technique

Date

14

Feb 2019

Time

11:00:00

Institut Pasteur, Rue du Docteur Roux, Paris, France

Address

Building: François Jacob

Location

2019-02-14 11:00:00 2019-02-14 12:00:00 Europe/Paris MicroOmiX : Enabling droplet microfluidics technologies SINGLE CELL PROTEOMICS TO SINGLE CELL SECRETOMICS Droplet-based microfluidics allow the reliable production of micrometer-sized incubators in which individual cells can be isolated and manipulated at high throughput rates. Such microfluidics-based technologies have been […] Institut Pasteur, Rue du Docteur Roux, Paris, France Pierre-Henri Commere pierre-henri.commere@pasteur.fr

Speakers

Sylvain Ursuegi

(faculté de Pharmacie Illkirch )

About

SINGLE CELL PROTEOMICS TO SINGLE CELL SECRETOMICS

Droplet-based microfluidics allow the reliable production of micrometer-sized incubators in which individual cells can be isolated and manipulated at high
throughput rates. Such microfluidics-based technologies have been successfully interfaced with RNA-Seq enabling the analysis of cell heterogeneity at the
transcriptome level. However, those technologies still suffer from serious limitations linked to the lack of efficient method for in-droplet biomarker capture and purification preventing the possibility to perform multi-omic investigations (transcriptomic/proteomic).
MicroOmiX addresses these limitations by combining two proprietary technologies:

- a highly efficient and robust capture system based on functional surfactant
  allowing the scavenging of a wide range of biomarkers;
- a droplet washing technology based on an inversion process enabling the
  separation of captured biomarkers from contaminants and other cell remnants

The detection of purified biomarkers can then be performed using current analyzer:

a cytometer for a targeted approach (up to 30 RNA/protein biomarkers) or
a sequencer for a non-targeted transcriptomic strategy combined with targeted proteomic.
[gview file=”https://research.pasteur.fr/wp-content/uploads/2019/02/research_pasteur-microomix-enabling-droplet-microfluidics-technologies-affiche-micro-fluidic-seminar.pdf”]

Location

Building: François Jacob
Address: Institut Pasteur, Rue du Docteur Roux, Paris, France

Published on: 11 Feb 2019

(function($) { /* * render_map * * This function will render a Google Map onto the selected jQuery element * * @type function * @date 8/11/2013 * @since 4.3.0 * * @param $el (jQuery element) * @return n/a */ function render_map( $el ) { var MY_MAPTYPE_ID = 'custom_style'; var featureOpts = [{"stylers":[{"hue":"#0088ff"}]}]; var styledMapOptions = { name: 'Custom Style' }; var customMapType = new google.maps.StyledMapType(featureOpts, styledMapOptions); // var var $markers = $el.find('.marker'); // vars var args = { zoom : 16, scrollwheel : false, center : new google.maps.LatLng(0, 0), mapTypeId : google.maps.MapTypeId.ROADMAP, mapTypeId : MY_MAPTYPE_ID }; // create map var map = new google.maps.Map( $el[0], args); map.mapTypes.set(MY_MAPTYPE_ID, customMapType); // add a markers reference map.markers = []; // add markers $markers.each(function(){ add_marker( $(this), map ); }); // center map center_map( map ); } /* * add_marker * * This function will add a marker to the selected Google Map * * @type function * @date 8/11/2013 * @since 4.3.0 * * @param $marker (jQuery element) * @param map (Google Map object) * @return n/a */ function add_marker( $marker, map ) { // var var latlng = new google.maps.LatLng( $marker.attr('data-lat'), $marker.attr('data-lng') ); //var image = 'https://research.pasteur.fr/wp-content/themes/biologyx2/images/logomap.png'; var myIcon = new google.maps.MarkerImage('https://research.pasteur.fr/wp-content/themes/biologyx2/images/map-pin.png', null, null, null, new google.maps.Size(32,32)); // create marker var marker = new google.maps.Marker({ position : latlng, map : map, icon : myIcon }); // add to array map.markers.push( marker ); // if marker contains HTML, add it to an infoWindow if( $marker.html() ) { // create info window var infowindow = new google.maps.InfoWindow({ content : $marker.html() }); // show info window when marker is clicked google.maps.event.addListener(marker, 'click', function() { infowindow.open( map, marker ); }); } } /* * center_map * * This function will center the map, showing all markers attached to this map * * @type function * @date 8/11/2013 * @since 4.3.0 * * @param map (Google Map object) * @return n/a */ function center_map( map ) { // vars var bounds = new google.maps.LatLngBounds(); // loop through all markers and create bounds $.each( map.markers, function( i, marker ){ var latlng = new google.maps.LatLng( marker.position.lat(), marker.position.lng() ); bounds.extend( latlng ); }); // only 1 marker? if( map.markers.length == 1 ) { // set center of map map.setCenter( bounds.getCenter() ); map.setZoom( 15 ); } else { // fit to bounds map.fitBounds( bounds ); } } /* * document ready * * This function will render each map when the document is ready (page has loaded) * * @type function * @date 8/11/2013 * @since 5.0.0 * * @param n/a * @return n/a */ $(document).ready(function(){ $('.acf-map').each(function(){ render_map( $(this) ); }); }); })(jQuery);