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© Research
Event

Dept Microbiology | Seminar “Cyclic nucleotide signaling in prokaryotic immune defenceGenome wide identification of functions essential for bypass of PBPs by L,D-transpeptidases in Escherichia coli”

Scientific Fields
Diseases
Organisms
Applications
Technique
Date
18
Nov 2022
Time
11:30:00
Institut Pasteur, Rue du Docteur Roux, Paris, France
Address
Room: AUDITORIUM CENTRE FRANCOIS JACOB
Location
2022-11-18 11:30:00 2022-11-18 12:30:00 Europe/Paris Dept Microbiology | Seminar “Cyclic nucleotide signaling in prokaryotic immune defenceGenome wide identification of functions essential for bypass of PBPs by L,D-transpeptidases in Escherichia coli” SEMINAR DEPT MICROBIOLOGY   Friday, November 18th 2022 at 11:30 am Auditorium Centre François Jacob   Malcolm White University of St Andrews, Scotland     Invited by Simonetta Gribaldo    (simonetta.gribaldo@pasteur.fr)   “Cyclic nucleotide […] Institut Pasteur, Rue du Docteur Roux, Paris, France Simonetta Gribaldo simonetta.gribaldo@pasteur.fr

Speakers

Malcolm White
(University of St Andrews, Scotland)

About

SEMINAR DEPT MICROBIOLOGY

 

Friday, November 18th 2022 at 11:30 am

Auditorium Centre François Jacob

 

Malcolm White

University of St Andrews, Scotland

 

 

Invited by Simonetta Gribaldo   

(simonetta.gribaldo@pasteur.fr)

 

“Cyclic nucleotide signaling in prokaryotic immune defence”

Abstract:

Prokaryotic cells uses a diverse array of cyclic nucleotide second messengers to coordinate defence against invading mobile genetic elements (MGE). Type III CRISPR systems detect foreign RNA and generate cyclic oligoadenylate (cOA) second messengers that activate powerful degradative nucleases, while CBASS (Cyclic nucleotide based antiphage signalling systems) make a wide range of cyclic di- and tri-nucleotides that frequently activate abortive infection (cell death) pathways. This talk will focus on the mechanisms of cyclic-nucleotide signalling in anti-viral defence, focussing on two examples under study in our lab. The first is a prophage-encoded type III CRISPR system from Vibrio cholerae that generates a cyclic triadenylate (cA3) signal, activating a powerful NucC DNA endonuclease effector. The second is a bacterial CBASS defence pathway that also functions via a cA3 second messenger, which activates an effector with an enzymatic TIR domain for degradation of cellular NAD+. This CBASS defence involves the assembly of a highly unusual and visually striking effector complex structure. The two pathways can be “mixed and matched” and most likely both operate via abortive infection, highlighting the conserved paradigms for cyclic nucleotide based prokaryotic defence systems.

Location

Room: AUDITORIUM CENTRE FRANCOIS JACOB
Address: Institut Pasteur, Rue du Docteur Roux, Paris, France