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© Research
Event

Department of Structural Biology and Chemistry seminar: Dr. Olivier Lambert, Antibiotic resistance and contribution of Multidrug Efflux Pumps: Structural and functional insights.

Scientific Fields
Diseases
Organisms
Applications
Technique
Date
21
Jan 2020
Time
14:00:00
28 Rue du Dr Roux, 75015 Paris, France
Address
Building: François Jacob Room: Auditorium François Jacob
Location
2020-01-21 14:00:00 2020-01-21 15:30:00 Europe/Paris Department of Structural Biology and Chemistry seminar: Dr. Olivier Lambert, Antibiotic resistance and contribution of Multidrug Efflux Pumps: Structural and functional insights. Department of Structural Biology and Chemistry SEMINAR TUESDAY 21 JANUARY 2020 at 2:00 pm  AUDITORIUM CENTRE F. JACOB room- CFJ RdC 17c Dr. Olivier Lambert Architecture de complexes membranaires et processus cellulaires CBMN Bordeaux […] 28 Rue du Dr Roux, 75015 Paris, France Nadia Izadi-Pruneyre nadia.izadi@pasteur.fr

About

Department of Structural Biology and Chemistry
SEMINAR

TUESDAY 21 JANUARY 2020 at 2:00 pm

 AUDITORIUM CENTRE F. JACOB room- CFJ RdC 17c

Dr. Olivier Lambert
Architecture de complexes membranaires et processus cellulaires
CBMN Bordeaux

 

 

Antibiotic resistance and contribution of Multidrug Efflux Pumps: Structural and functional insights.

The dangerous increase of resistant pathogenic bacteria (including Enterococcus faecium, Staphylococcus aureu, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species), to most of available antibiotics emerges as a worldwide public health concern. It is urgent to develop original solution and focus on unconventional targets. Active efflux is one of the four main mechanisms by which bacteria exhibit resistance to antibiotic.

In Gram-negative bacteria, tripartite efflux pumps spanning the cell envelope can mediate the efflux of a wide variety of antimicrobial compounds, and participate to the antibiotic resistance. These efflux systems share a similar structural architecture composed of an inner membrane transporter and an outer membrane channel bridged by a periplasmic adaptor protein. The prototypical and clinically relevant efflux pump from Pseudomonas aeruginosa, MexAB-OprM is involved in the transport of drugs from the periplasm to the extracellular medium through the use of the proton gradient.

We have developed a method to reconstitute a tripartite assembly from native components using lipid nanodiscs. MexB and OprM inserted in lipid nanodisc self-assembled in the presence of MexA. The structure of tripartite system has been studied by electron microscopy [1]. High resolution single particle cryo-EM analysis complemented with transport activity measurements shed new light on key factors eliciting the functional drug extrusion.

[1] Daury, L., Orange, F., Taveau, J. C., Verchère, A., Monlezun, L., Gounou, C., … & Lambert, O. (2016). Tripartite assembly of RND multidrug efflux pumps. Nature communications, 7.

 

Contact : Nadia Izadi-Pruneyre
Bioinformatique Structurale
Nadia.izadi@pasteur.fr

INSTITUT PASTEUR - 28, rue du Docteur Roux – 75015 Paris

							

Location

Building: François Jacob
Room: Auditorium François Jacob
Address: 28 Rue du Dr Roux, 75015 Paris, France