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  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
30
Jun 2022
Ending Date
06
Jul 2022
Time
09:00:00
28 Rue du Docteur Roux, Paris, France
Address
2022-06-30 09:00:00 2022-07-06 18:00:00 Europe/Paris Single cell gene expression and beyond Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […] 28 Rue du Docteur Roux, Paris, France Valentina Libri valentina.libri@pasteur.fr

Course Overview

The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions.

Program
This 5-days course will cover the following topics:

Overview of technologies for single cells omics and fundamentals of NGS (0.5 day); Separation of single cells by FACS (0.5 day); Single cell RNA prep by droplet-based assays (10xGenomics) (0.5 day); Preparation and quantification of single cell RNA-Seq libraries (1 day); Introduction to microfluidics-based approaches for single cell profiling (0.5 day); Basics of single cell transcriptome data analysis using state-of-the-art data analysis pipelines (2 days).

Quality Controls, best practices and “tips and tricks” will be discussed.

Audience
Scientists, students and Core Facility staff interested in applying advanced single-cell transcriptome sequencing in their research are welcome. Basic knowledge of molecular biology and basics in “R” are desirable

Online registration: webcampus.pasteur.fr/ip/single-cel

Co-directors:
Valentina LIBRI
Milena HASAN

Deadline for application: 30th April 2022
Attendees: 12 participants
Contact: formation@pasteur.fr

Location

Location: 28 Rue du Docteur Roux, Paris, France