Course Overview
The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions.
Program
This 5-days course will cover the following topics:
Overview of technologies for single cells omics and fundamentals of NGS (0.5 day); Separation of single cells by FACS (0.5 day); Single cell RNA prep by droplet-based assays (10xGenomics) (0.5 day); Preparation and quantification of single cell RNA-Seq libraries (1 day); Introduction to microfluidics-based approaches for single cell profiling (0.5 day); Basics of single cell transcriptome data analysis using state-of-the-art data analysis pipelines (2 days).
Quality Controls, best practices and “tips and tricks” will be discussed.
Audience
Scientists, students and Core Facility staff interested in applying advanced single-cell transcriptome sequencing in their research are welcome. Basic knowledge of molecular biology and basics in “R” are desirable
Online registration: webcampus.pasteur.fr/ip/single-cel
Co-directors:
Valentina LIBRI
Milena HASAN
Deadline for application: 30th April 2022
Attendees: 12 participants
Contact: formation@pasteur.fr
Location
Location: 28 Rue du Docteur Roux, Paris, France
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/*
* render_map
*
* This function will render a Google Map onto the selected jQuery element
*
* @type function
* @date 8/11/2013
* @since 4.3.0
*
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* @return n/a
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function render_map( $el ) {
var MY_MAPTYPE_ID = 'custom_style';
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var styledMapOptions = {
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var customMapType = new google.maps.StyledMapType(featureOpts, styledMapOptions);
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scrollwheel : false,
center : new google.maps.LatLng(0, 0),
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/*
* add_marker
*
* This function will add a marker to the selected Google Map
*
* @type function
* @date 8/11/2013
* @since 4.3.0
*
* @param $marker (jQuery element)
* @param map (Google Map object)
* @return n/a
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function add_marker( $marker, map ) {
// var
var latlng = new google.maps.LatLng( $marker.attr('data-lat'), $marker.attr('data-lng') );
//var image = 'https://research.pasteur.fr/wp-content/themes/biologyx2/images/logomap.png';
var myIcon = new google.maps.MarkerImage('https://research.pasteur.fr/wp-content/themes/biologyx2/images/map-pin.png', null, null, null, new google.maps.Size(32,32));
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google.maps.event.addListener(marker, 'click', function() {
infowindow.open( map, marker );
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/*
* center_map
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*
* @type function
* @date 8/11/2013
* @since 4.3.0
*
* @param map (Google Map object)
* @return n/a
*/
function center_map( map ) {
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var latlng = new google.maps.LatLng( marker.position.lat(), marker.position.lng() );
bounds.extend( latlng );
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map.setZoom( 15 );
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map.fitBounds( bounds );
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/*
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*
* @type function
* @date 8/11/2013
* @since 5.0.0
*
* @param n/a
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$(document).ready(function(){
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