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© Research
Publication : STAR protocols

A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy.

Team: Fungal Biology and Pathogenicity
Member: Thierry Mourer
Member: Martin Sachse
Member: Anastasia Gazi
Member: Christophe D’Enfert
Member: Sophie Bachellier-Bassi
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in STAR protocols - 01 Jul 2022

Mourer T, Sachse M, Gazi AD, d'Enfert C, Bachellier-Bassi S,

Link to Pubmed [PMID] – 35779259

Link to DOI – S2666-1667(22)00394-X10.1016/j.xpro.2022.101514

STAR Protoc 2022 Jul; 3(3): 101514

This protocol describes how to analyze C. albicans biofilm using transmission electron microscopy. We present two approaches to observe the ultrastructure of fungal cells within unperturbed biofilms, as well as an immunogold labeling procedure. This approach maintains the architecture of the fungal biofilm close to its native state by growing C. albicans biofilm on a plastic surface. After the freeze substitution procedure, classical transmission electron microscopy or electron tomography will allow the ultrastructural analysis of the microbial community.