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  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • MD-PhD Student
  • Medical Staff
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Starting Date
07
Feb 2022
Keywords
11 Keywords
Status
Completed
Members
1
Structures
4

About

Immunology at work – resource center

Whether you want to review the basics, gain more
in-depth knowledge, or discover the latest research tools, this resource hub contains rich and reliable technical content, designed for new and experienced life scientists alike, exploring the field of immunology.
Handbook PDF => flow-cytometry-protocols-handbook

    • Sample preparation
      Isolation of human PBMCs from whole blood
      Mouse bone marrow cell isolation
      Mouse spleen cell isolation protocol
      Whole blood staining protocol for flow cytometry analysis Red blood cell lysis
    • Immune cell stimulation
      In vitro differentiation of macrophages from monocytes Stimulation of cytokine production in immune cells
      T cell activation
    • Phenotypic analysis
      Staining cell surface targets for flow cytometry
      Staining intracellular antigens for flow cytometry Intracellular staining guide for human and mouse cytokines Compensation
      Panel design
      Blocking non-specific binding with CellBlox™ Blocking Buffer
    • Cell viability and apoptosis
      Cell viability
      Annexin V staining for apoptosis detection Camptothecin induced apoptosis protocol
    • Cell cycle
      DNA content analysis in living cells
      DNA content in fixed cells
    • Cell proliferation
      Cell proliferation assay
      Generational analysis of T cell proliferation