Présentation
NMR spectroscopy
800 MHz NMR spectrometer installed in Pasteur
Three NMR spectrometers with 11.7, 14 and 18 Tesla magnets (500 MHz, 600 MHz and 800Mhz), well suited for study of biomolecules are currently in use in the lab. The 500Mhz was upgraded in 2009. The 600Mhz has been upgraded in 2016, the 800Mhz has installed in 2016. Both spectrometers are equipped with four channels (1H, 13C, 15N, 2H).
The Varian NMR System 500 spectrometer is equipped with several probes (triple resonance, broadband, indirect detection).
The 600Mhz and 800Mhz spectrometers are equipped with the latest generation 5 mm triple resonance 1H{13C/15N} cryoprobe with increased sensitivity for both 1H and 13C.
The wet-lab, other facilities
We are currently using different tools for the expression, purification and biochemical characterisation of proteins.
The lab’s facilities include absorption and fluorescence spectrometers.
We have the practical knowledge and an access to numerous technical resources available at several Institut Pasteur’s technological platforms. Among them, the Biophysics Platform (microcalorimetry, circular dichroïsm, infrared spectroscopy, fluorescence, surface plasmon resonance…), Protein Microsequencing and Analysis, Production of Recombinant Proteins.
