Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR.

Lien vers Pubmed [PMID] – 27316653

Lien DOI – S0167-7012(16)30144-010.1016/j.mimet.2016.06.015

J. Microbiol. Methods 2016 08; 127(): 160-163

Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.