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© A. Alanio, E. Perret
Prolifération de Cryptococcus neoformans dans des macrophages murins.
Publication : Journal of microbiological methods

Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of microbiological methods - 14 Jun 2016

Alanio A, Sturny-Leclère A, Benabou M, Guigue N, Bretagne S

Link to Pubmed [PMID] – 27316653

J. Microbiol. Methods 2016 Aug;127:160-3

Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.

https://www.ncbi.nlm.nih.gov/pubmed/27316653