Specific metabolic changes occurring during biofilm formation were hypothesized to lead to the production of biofilm-associated molecules and biofilm-specific functions. The identification of biofilm-specific metabolites could reveal new aspects of biofilm biology, and lead to novel strategies to diagnose, fight, or use bacterial biofilms.
Recent articles on this topic
• Szczesny, M., C. Beloin and J.-M. Ghigo (2018). Increased osmolarity in biofilm triggers RcsB-dependent lipid A palmitoylation in Escherichia coli. mBio. In press.
• Létoffé, S., S. Chalabev, J. Dugay, F. Stressmann, B. Audrain, J.C. Portais, F. Letisse, and J.M. Ghigo. (2017). Biofilm microenvironment induces a widespread adaptive amino-acid fermentation pathway conferring strong fitness advantage in Escherichia coli. PLoS Genetics. May 19;13(5) doi: 10.1371.
Review on this topic
• Beloin, C. and J.M. Ghigo. (2005). Finding gene-expression patterns in bacterial biofilms. Trends in Microbiology 13:16-9.
Ghigo, J.M. (2003). Are there biofilm-specific physiological pathways beyond a reasonable doubt? Research in Microbiology
ANAEROBIC BIOFILMS AND BACTERIAL COMMUNITIES
Although many clinically and ecologically relevant processes are known to be performed by biofilm communities composed of strict and facultative anaerobic bacteria, biofilms were mainly studied in aerobic conditions. In collaboration with the Laboratory of Anaerobic Bacterial Pathogens at the Institut Pasteur, we use in vitro anaerobic culture systems combined with genetic and genomic approaches to study the molecular basis of biofilm formation by anaerobic commensal and pathogenic bacteria.