Detection and countermeasures against bacterial toxins
Bacterial protein toxins are molecular nanodevices endowed with the capacity of inducing host specific unparalleled pathophysiological effects. Therefore, major challenges in toxinology include the development of countermeasures other than vaccination against bacterial protein toxins […]
Biophysical characterization of the translocation process of Bordetella pertussis adenylate cyclase toxin CyaA
This project aims to describe the hydrodynamic and thermodynamic properties of the CyaA toxin and the conformational changes it undergoes during its translocation process across the lipid membranes of Bordetella pertussis (secretion) and of […]
We are interested in investigating the effects of molecular crowding, i.e., excluded volume effects, mimicking the effects of high concentrations of macromolecular solutes found in biological fluids on biochemical properties of proteins. In particular, […]
Clinical development of cancer vaccines
CyaA-based therapeutic cancer vaccines Based on our discovery that a bacterial protein, the adenylate cyclase (CyaA), binds to dendritic cells (DCs), we have developed a highly efficient vector capable of targeting a wide range […]
CyaA secretion, folding and translocation across membrane
CyaA, a 1706 residue-long protein, is one of the major virulence factors produced by B. pertussis and plays an important role in the early stages of respiratory tract colonization. This toxin uses an original […]
Regulatory roles of signaling Nucleotides
Other research projects are aimed at exploring the regulatory role of different nucleotides such ppGpp and pppGpp that are both thought to act as second messengers in many complex cellular responses to nutritional […]
In vivo analysis of protein-protein interactions
A second main line of research of the team is focused on the characterization of protein-protein interactions by exploiting a bacterial two-hybrid screening technology (BACTH) that we have previously developed (Karimova et al., […]
Recombinant CyaA toxin as an innovative vaccine vehicle
From an applied perspective, we have shown in collaboration with the team of C. Leclerc, (Immune Regulation and Vaccinology, Institut Pasteur) that CyaA can be engineered to serve as a potent non-replicating vector […]
Structure-function studies of the CyaA toxin
The CyaA toxin is a 1706 residues-long bifunctional protein (Figure 1): the calmodulin-activated, catalytic domain is located in the 400 amino-proximal residues, whereas the carboxy-terminal 1306 residues are responsible for the binding of […]
2022Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium., BMC Biol 2022 08; 20(1): 176.
2021A High-Affinity Calmodulin-Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells., Adv Sci (Weinh) 2021 05; 8(9): 2003630.
2021Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Vaccine Development and Other Biotechnological Purposes., Toxins (Basel) 2021 Jan; 13(2): .
2019Post-translational acylation controls the folding and functions of the CyaA RTX toxin., FASEB J 2019 09; 33(9): 10065-10076.
2019Editorial: Pathogens and disease special issue ETOX18, Pathog Dis 2019 03;77(2).
2019The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro., Toxins (Basel) 2019 02; 11(2): .
2018Differential regulation of actin-activated nucleotidyl cyclase virulence factors by filamentous and globular actin, PLoS ONE 2018;13(11):e0206133.
2018The extreme C terminus of the effector ExoY is crucial for binding to its eukaryotic activator, F-actin, J. Biol. Chem. 2018 12;293(51):19785-19796.
2018Bioengineering of Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy, Toxins (Basel) 2018 Jul;10(7).
2018Compartmentalized Cyclic AMP Production by the and Adenylate Cyclase Toxins Differentially Affects the Immune Synapse in T Lymphocytes, Front Immunol 2018;9:919.
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