Présentation
Post translational modifications (PTMs) are known to play pivotal roles in cellular physiology and disease, but only a few of the over 300 currently known modifications are extensively investigated in proteomic studies. The most common PTMs are phosphorylation, glycosylation, ubiquitination, sumoylation, methylation, acetylation and disulfide bridge formation. To identify and localize the modification, we use a combination of different strategies for sample preparation, including enrichment or digestion, and MS fragmentation techniques (HCD, ETD, CID), for example.