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© Research
Publication : International journal of food microbiology

Use of pulsed field gel electrophoresis to compare large DNA-restriction fragments of Listeria monocytogenes strains belonging to serogroups 1/2 and 3

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in International journal of food microbiology - 01 Dec 1991

Buchrieser C, Brosch R, Rocourt J

Link to Pubmed [PMID] – 1790106

Int. J. Food Microbiol. 1991 Dec;14(3-4):297-304

Thirty-five Listeria monocytogenes strains belonging to serogroups 1/2 and 3 and isolated from various origins were characterized by whole cellular DNA restriction patterns using low-frequency cleavage enzymes and pulsed field gel electrophoresis. Seventeen restriction profiles were detected with ApaI, 18 with SmaI and 15 with NotI, the combination of these patterns allowing one to define at least 24 distinct groups within the 35 strains. The significant genomic diversity pointed out by this method can be of value in the epidemiological fingerprinting of L. monocytogenes.

http://www.ncbi.nlm.nih.gov/pubmed/1790106