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© Therese Couderc, Marc Lecuit
Publication : Biologicals : journal of the International Association of Biological Standardization

Unbiased analysis by high throughput sequencing of the viral diversity in fetal bovine serum and trypsin used in cell culture

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Biologicals : journal of the International Association of Biological Standardization - 22 Mar 2014

Gagnieur L, Cheval J, Gratigny M, Hébert C, Muth E, Dumarest M, Eloit M

Link to Pubmed [PMID] – 24661556

Biologicals 2014 May;42(3):145-52

Fetal bovine serum (FBS) and trypsin are reagents used in cell culture and have been the source of viral contamination of pharmaceutical products. We performed high throughput sequencing (HTS) of two pools of commercial batches of FBS and three commercial batches of trypsin. Taxonomies were assigned by comparing sequences of contigs and singletons to the entire NCBI nucleic acid and protein databases. The same major viral species were evidenced between batches of a given reagent but the proportion of viral reads among total reads varied markedly between samples (from 0.002% to 22.7%). In FBS, the sequences found were mainly from bovine viral diarrhea virus (BVDV) 1 to 3 and bovine parvovirus 3 (BPV3). The BVDV sequences derived from FBS showed only minor discrepancies with primers generally used for the screening of BVDV. Viral sequences in trypsin were mainly from porcine circovirus type 2. Other known viral sequences at lower read counts and potential new viral species (bovine parvovirus and bovine pegivirus) were evidenced. The load of some known and new viruses detected by HTS could be quantified by qPCR. Results of HTS provide a framework for evaluating the pertinence of control measures including the design of PCRs, bioassays and inactivation procedures.

http://www.ncbi.nlm.nih.gov/pubmed/24661556