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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Proceedings of the National Academy of Sciences of the United States of America

Three-dimensional architecture of actin filaments in Listeria monocytogenes comet tails

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Proceedings of the National Academy of Sciences of the United States of America - 04 Dec 2013

Jasnin M, Asano S, Gouin E, Hegerl R, Plitzko JM, Villa E, Cossart P, Baumeister W

Link to Pubmed [PMID] – 24306931

Proc. Natl. Acad. Sci. U.S.A. 2013 Dec;110(51):20521-6

The intracellular bacterial pathogen Listeria monocytogenes is capable of remodelling the actin cytoskeleton of its host cells such that “comet tails” are assembled powering its movement within cells and enabling cell-to-cell spread. We used cryo-electron tomography to visualize the 3D structure of the comet tails in situ at the level of individual filaments. We have performed a quantitative analysis of their supramolecular architecture revealing the existence of bundles of nearly parallel hexagonally packed filaments with spacings of 12-13 nm. Similar configurations were observed in stress fibers and filopodia, suggesting that nanoscopic bundles are a generic feature of actin filament assemblies involved in motility; presumably, they provide the necessary stiffness. We propose a mechanism for the initiation of comet tail assembly and two scenarios that occur either independently or in concert for the ensuing actin-based motility, both emphasizing the role of filament bundling.

http://www.ncbi.nlm.nih.gov/pubmed/24306931