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© Research
Publication : Bioinformatics

TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Bioinformatics - 18 Nov 2021

Dhenin J, Lima DB, Dupré M, Chamot-Rooke J,

Link to Pubmed [PMID] – 34792554

Link to DOI – 10.1093/bioinformatics/btab784

Bioinformatics 2021 Nov; ():

We present a new software-tool allowing an easy visualization of fragment ions and thus a rapid evaluation of key experimental parameters on the sequence coverage obtained for the MS/MS analysis of intact proteins. Our tool can process data obtained from various deconvolution and fragment assignment software.We demonstrate that TDFragMapper can rapidly highlight the experimental fragmentation parameters that are critical to the characterization of intact proteins of various size using top-down proteomics.TDFragMapper, a demonstration video and user tutorial are freely available for academic use at https://msbio.pasteur.fr/tdfragmapper; all data are thus available from the ProteomeXchange consortium (identifier PXD024643).Supplementary data are available at Bioinformatics online.

https://pubmed.ncbi.nlm.nih.gov/34792554