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© Deriano Lab / Institut Pasteur
Chromosomes métaphasiques d’une cellule lymphoïde cancéreuse présentant une amplification des gènes Igh et c-myc
Publication : Genes & Development

Synthetic lethality between PAXX and XLF in mammalian development

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Genes & Development - 01 Oct 2016

Gabriel Balmus, Ana C. Barros, Paul W.G. Wijnhoven, Chloé Lescale, Hélène Lenden Hasse, Katharina Boroviak, Carlos le Sage, Brendan Doe, Anneliese O. Speak, Antonella Galli, Matt Jacobsen, Ludovic Deriano, David J. Adams, Andrew N. Blackford and Stephen P. Jackson

Link to Pubmed [PMID] – 27798842

Genes Dev. 2016 Oct 1;30(19):2152-2157.

PAXX was identified recently as a novel nonhomologous end-joining DNA repair factor in human cells. To characterize its physiological roles, we generated Paxx-deficient mice. Like Xlf−/− mice, Paxx−/− mice are viable, grow normally, and are fertile but show mild radiosensitivity. Strikingly, while Paxx loss is epistatic with Ku80, Lig4, and Atm deficiency, Paxx/Xlf double-knockout mice display embryonic lethality associated with genomic instability, cell death in the central nervous system, and an almost complete block in lymphogenesis, phenotypes that closely resemble those of Xrcc4−/− and Lig4−/− mice. Thus, combined loss of Paxx and Xlf is synthetic-lethal in mammals.