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© Research
Publication :

Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in - 17 May 2019

Stella Diafa, Damien Evéquoz, Christian J. Leumann, and Marcel Hollenstein*

Methods Mol. Biol. 2019, 1973, 1-13

Chemical modification of nucleic acids can be achieved by the enzymatic polymerization of modified nucleoside triphosphates (dN*TPs). This approach obviates some of the requirements and drawbacks imposed by the more traditional solid-phase synthesis of oligonucleotides.

Here, we describe the protocol that is necessary to synthesize dN*TPs and evaluate their substrate acceptance by polymerases for their subsequent use in various applications including selection experiments to identify aptamers. The protocol is exemplified for a sugar-constrained nucleoside analog, 7ˈ,5ˈ-bc-TTP.