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© Research
Publication : The Biochemical journal

Synergistic role of nucleotides and lipids for the self-assembly of Shs1 septin oligomers.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Biochemical journal - 31 Jul 2020

Taveneau C, Blanc R, Péhau-Arnaudet G, Di Cicco A, Bertin A,

Link to Pubmed [PMID] – 32726433

Link to DOI [DOI] – 10.1042/BCJ20200199

Biochem J 2020 Jul; 477(14): 2697-2714

Budding yeast septins are essential for cell division and polarity. Septins assemble as palindromic linear octameric complexes. The function and ultra-structural organization of septins are finely governed by their molecular polymorphism. In particular, in budding yeast, the end subunit can stand either as Shs1 or Cdc11. We have dissected, here, for the first time, the behavior of the Shs1 protomer bound to membranes at nanometer resolution, in complex with the other septins. Using electron microscopy, we have shown that on membranes, Shs1 protomers self-assemble into rings, bundles, filaments or two-dimensional gauzes. Using a set of specific mutants we have demonstrated a synergistic role of both nucleotides and lipids for the organization and oligomerization of budding yeast septins. Besides, cryo-electron tomography assays show that vesicles are deformed by the interaction between Shs1 oligomers and lipids. The Shs1-Shs1 interface is stabilized by the presence of phosphoinositides, allowing the visualization of micrometric long filaments formed by Shs1 protomers. In addition, molecular modeling experiments have revealed a potential molecular mechanism regarding the selectivity of septin subunits for phosphoinositide lipids.

https://pubmed.ncbi.nlm.nih.gov/32726433