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© Melody Merle
Fluorescently labeled five day old gastruloid, a mouse embryonic stem-cell derived pseudo-embryo.
Publication : Methods in molecular biology (Clifton, N.J.)

Single mRNA Molecule Detection in Drosophila.

Team: Physics of Biological Function
Member: Thomas Gregor
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2018

Little SC, Gregor T,

Link to Pubmed [PMID] – 29130194

Link to DOI – 10.1007/978-1-4939-7213-5_8

Methods Mol Biol 2018 ; 1649(): 127-142

Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.