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© Melody Merle
Fluorescently labeled five day old gastruloid, a mouse embryonic stem-cell derived pseudo-embryo.
Publication : Methods in molecular biology (Clifton, N.J.)

Laser Ablation of Microtubule-Chromosome Attachment in Mouse Oocytes.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2018

Bennabi I, Manil-Ségalen M,

Link to Pubmed [PMID] – 29961264

Link to DOI – 10.1007/978-1-4939-8603-3_16

Methods Mol Biol 2018 ; 1818(): 153-161

Laser ablation is a powerful tool to study forces within biological systems. This technique has been extensively used to study mitotic spindle formation and chromosome segregation. This chapter describes laser ablation of microtubule-chromosome attachments coupled to fluorescence live microscopy and quantitative analysis of individual chromosome movement after microtubule severing. This method allows to gain insight into the organization and dynamics of the meiotic spindle and chromosomes in metaphase I mouse oocytes.

https://pubmed.ncbi.nlm.nih.gov/29961264