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© Research
Publication : Molecular immunology

Single chain antibody fragments for the selective targeting of antigens to dendritic cells

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular immunology - 10 Dec 2004

Demangel C, Zhou J, Choo AB, Shoebridge G, Halliday GM, Britton WJ

Link to Pubmed [PMID] – 15829289

Mol. Immunol. 2005 May;42(8):979-85

In order to target antigens (Ags) selectively to dendritic cells (DC), we derived single chain antibody fragments (scFvs) from NLDC-145 and N418, two monoclonal antibodies binding the mouse dendritic cell-restricted surface molecules DEC-205 and CD11c. Recombinant hexahistidine-tagged forms of the scFvs (scNLDC and scN418) were efficiently produced in a baculovirus expression system. Both scFvs bound DEC-205(+) Langerhans cells and CD11c(+) fetal skin-derived dendritic cells (FSDCs) comparably to their parental antibodies. Immunization of C57BL/6 mice with a DNA vaccine encoding a model protein antigen fused to scNLDC stimulated specific immune responses in both the humoral and cellular compartments, in contrast to DNA vaccines expressing scN418-targeted or untargeted antigen. Our results show that antigen targeting to DCs via a DEC-205 binding scFv leads to enhanced immunogenicity. Further, this work suggests that scFvs fused to protein antigens and delivered as DNA vaccines may provide a generic means for delivering vaccinal molecules to selected cell populations.

http://www.ncbi.nlm.nih.gov/pubmed/15829289