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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Roadmap for annotating transposable elements in eukaryote genomes

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2012

Permal E, Flutre T, Quesneville H

Link to Pubmed [PMID] – 22367865

Methods Mol. Biol. 2012;859:53-68

Current high-throughput techniques have made it feasible to sequence even the genomes of non-model organisms. However, the annotation process now represents a bottleneck to genome analysis, especially when dealing with transposable elements (TE). Combined approaches, using both de novo and knowledge-based methods to detect TEs, are likely to produce reasonably comprehensive and sensitive results. This chapter provides a roadmap for researchers involved in genome projects to address this issue. At each step of the TE annotation process, from the identification of TE families to the annotation of TE copies, we outline the tools and good practices to be used.

https://www.ncbi.nlm.nih.gov/pubmed/22367865