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© Marion Coolen
Radial glia neural stem cells of the adult zebrafish telencephalon electroporated with a membrane tagged GFP
Publication : Zebrafish

Photoactivation of the CreER T2 recombinase for conditional site-specific recombination with high spatiotemporal resolution

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Zebrafish - 01 Jun 2010

Sinha DK, Neveu P, Gagey N, Aujard I, Le Saux T, Rampon C, Gauron C, Kawakami K, Leucht C, Bally-Cuif L, Volovitch M, Bensimon D, Jullien L, Vriz S

Link to Pubmed [PMID] – 20441524

Zebrafish 2010 Jun;7(2):199-204

We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).

http://www.ncbi.nlm.nih.gov/pubmed/20441524