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© Melody Merle
Fluorescently labeled five day old gastruloid, a mouse embryonic stem-cell derived pseudo-embryo.
Publication : Cell reports

Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Cell reports - 22 Mar 2022

Singh AP, Wu P, Ryabichko S, Raimundo J, Swan M, Wieschaus E, Gregor T, Toettcher JE,

Link to Pubmed [PMID] – 35320726

Link to DOI – S2211-1247(22)00284-410.1016/j.celrep.2022.110543

Cell Rep 2022 03; 38(12): 110543

Developmental patterning networks are regulated by multiple inputs and feedback connections that rapidly reshape gene expression, limiting the information that can be gained solely from slow genetic perturbations. Here we show that fast optogenetic stimuli, real-time transcriptional reporters, and a simplified genetic background can be combined to reveal the kinetics of gene expression downstream of a developmental transcription factor in vivo. We engineer light-controlled versions of the Bicoid transcription factor and study their effects on downstream gap genes in embryos. Our results recapitulate known relationships, including rapid Bicoid-dependent transcription of giant and hunchback and delayed repression of Krüppel. In addition, we find that the posterior pattern of knirps exhibits a quick but inverted response to Bicoid perturbation, suggesting a noncanonical role for Bicoid in directly suppressing knirps transcription. Acute modulation of transcription factor concentration while recording output gene activity represents a powerful approach for studying developmental gene networks in vivo.

https://pubmed.ncbi.nlm.nih.gov/35320726