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© Research
Publication : Mammalian genome : official journal of the International Mammalian Genome Society

Nonpermissiveness for mouse embryonic stem (ES) cell derivation circumvented by a single backcross to 129/Sv strain: establishment of ES cell lines bearing the Omd conditional lethal mutation

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Mammalian genome : official journal of the International Mammalian Genome Society - 01 Dec 1998

Kress C, Vandormael-Pournin S, Baldacci P, Cohen-Tannoudji M, Babinet C

Link to Pubmed [PMID] – 9880667

Mamm. Genome 1998 Dec;9(12):998-1001

The inbred mouse strain DDK carries a conditional early embryonic lethal mutation that is manifested when DDK females are crossed to males of other inbred strains but not in the corresponding reciprocal crosses. It has been shown that embryonic lethality could be assigned to a single genetic locus called Ovum mutant (Om), on Chromosome (Chr) 11 near Syca 1. In the course of our study of the molecular mechanisms underlying the embryonic lethality, we were interested in deriving an embryonic stem cell bearing the Om mutation in the homozygous state (Omd/Omd). However, it turned out that DDK is nonpermissive for ES cell establishment, with a standard protocol. Here we show that permissiveness could be obtained using Omd/Omd blastocysts with a 75% 129/Sv and 25% DDK genetic background. Several germline-competent Omd/Omd ES cell lines have been derived from blastocysts of this genotype. Such a scenario could be extended to the generation of ES cell lines bearing any mutation present in an otherwise nonpermissive mouse strain.

http://www.ncbi.nlm.nih.gov/pubmed/9880667