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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Nature

Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature - 20 Sep 1984

Ebright RH, Cossart P, Gicquel-Sanzey B, Beckwith J

Link to Pubmed [PMID] – 6090927

Nature 1984 Sep 20-26;311(5983):232-5

Three mutations that alter the DNA sequence specificity of the catabolite gene activator protein (CAP) from AA-TGTGA–T—TCA-ATW to AA-TGTAA–T—TCA-ATW have been isolated. All three mutations affect the same amino acid of CAP, glutamic acid 181. We propose that it is this amino acid of CAP that makes contacts with base pairs 7 and 16 of the symmetrical recognition site.

http://www.ncbi.nlm.nih.gov/pubmed/6090927