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© Research
Publication : Journal of infection in developing countries

Multilocus VNTR analysis of Mycobacterium ulcerans strains isolated in Côte d’Ivoire

Team: Archived: Group: Sylvain Brisse
Team: Microbial Evolutionary Genomics
Team: Malaria Infection & Immunity
Member: Sylvain Brisse
Member: Jean-Michel Thiberge
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of infection in developing countries - 01 Feb 2011

Coulibaly-N'Golo GM, Ekaza E, Coulibaly B, Aka N, N'guessan RK, Thiberge JM, Caro V, Brisse S, Bretin-Dosso M

Link to Pubmed [PMID] – 21330742

J Infect Dev Ctries 2011 Feb;5(1):59-63

INTRODUCTION: Buruli ulcer, caused by Mycobacterium ulcerans, is endemic in more than 30 countries worldwide, with Côte d’Ivoire being among the most affected countries.

METHODOLOGY: We used seven variable number of tandem repeats (VNTR) markers and analyzed 114 samples from 11 Ivorian localities consisting of 33 bacterial strains and 81 clinical samples. Complete data sets at loci 1, 6, 9 and 33 were obtained for 18 of these strains (n = 15) and samples (n = 3) collected in each of the localities.

RESULTS: All the strains had allelic profile [3113], corresponding to the previously described Atlantic Africa genotype.

CONCLUSION: Sequencing of PCR products at all loci showed no variation in sequence or repeat number, underlining the genetic monomorphism of M. ulcerans in Côte d’Ivoire.