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© Research
Publication : 2021 IEEE 18th International Symposium on Biomedical Imaging (ISBI)

Morphological Reconstruction of Detached Dendritic Spines via Geodesic Path Prediction

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in 2021 IEEE 18th International Symposium on Biomedical Imaging (ISBI) - 13 Apr 2021

Sammit Jain, Suvadip Mukherjee, Lydia Danglot, Jean-Christophe Olivo-Marin

Link to DOI – 10.1109/ISBI48211.2021.9433981

Morphological reconstruction of dendritic spines from fluorescent microscopy is a critical open problem in neuro-image analysis. Existing segmentation tools are ill-equipped to handle thin spines with long, poorly illuminated neck membranes. We address this issue, and introduce an unsupervised path prediction technique based on a stochastic framework which seeks the optimal solution from a path-space of possible spine neck reconstructions. Our method is specifically designed to reduce bias due to outliers, and is adept at reconstructing challenging shapes from images plagued by noise and poor contrast. Experimental analyses on two photon microscopy data demonstrate the efficacy of our method, where an improvement of 12.5% is observed over the state-of-the-art in terms of mean absolute reconstruction error.

https://ieeexplore.ieee.org/abstract/document/9433981