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© Institut Pasteur
Spirochète : bactérie hélicoïdale, flexible et ondulante de longueur variable, non colorable par la coloration de Gram, très mobile (endoflagelles). Trois familles : Spirochaetaceae, Leptospiraceae, et Brachyspiraceae. Principaux genres pathogènes pour l'homme : Borrelia (Borrelia burgdorferi cause de la maladie de Lyme), Treponema (Treponema pallidum cause de la syphillis), Leptospira (Leptospira interrogans serovar icterohaemorrhagiae cause de la maladie de Weil). Image colorisée.
Publication : Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

Molecular characterization of the first leptospires isolated from goats in Brazil.

Team: Biology of Spirochetes
Reference Center: Leptospirosis
Member: Mathieu Picardeau
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] - 01 Jan 2014

Lilenbaum W, Kremer F, Ristow P, Dellagostin O, Bourhy P, Hartskeerl R, Vasconcellos S,

Link to Pubmed [PMID] – 25763063

Braz J Microbiol 2014 ; 45(4): 1527-30

Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca.