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© Research
Publication : Virology journal

Molecular characterization of Orf virus in goats in Gabon, Central Africa

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Virology journal - 13 May 2016

Maganga GD, Relmy A, Bakkali-Kassimi L, Ngoubangoye B, Tsoumbou T, Bouchier C, N'Dilimabaka N, Leroy EM, Zientara S, Berthet N

Link to Pubmed [PMID] – 27178401

Virol. J. 2016;13:79

BACKGROUND: Orf or contagious ecthyma is a zoonotic viral infection with a potential serious health threat for the small ruminants industry as well as humans. It is currently emerging in new territories.

RESULTS: Eight suspected clinical cases of pustular dermatitis in goats occurred in the rural area of Tebe, in south-eastern Gabon, in January 2013. The orf virus (ORFV) was detected by high-throughput sequencing on sera, buccal swabs and scab pool samples. It was confirmed in six out of eight sick goats by using specific PCR targeting the major envelope protein (B2L) and the orf virus interferon resistance (VIR) genes. Phylogenetic analysis revealed that the Gabonese strain and South Korean strains evolved from a common ancestor, suggesting an Asian origin of the ORFV’ Gabonese strain.

CONCLUSIONS: This study provides the molecular detection of the ORFV strain involved in the cases of pustular dermatitis in goats and highlights its circulation in Gabon.

http://www.ncbi.nlm.nih.gov/pubmed/27178401