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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Microchannels for the Study of T Cell Immunological Synapses and Kinapses

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2017

Moreau HD, Bousso P, Lennon-Duménil AM

Link to Pubmed [PMID] – 28255711

Methods Mol. Biol. 2017;1584:347-354

T Cells can form very stable (synapses) or very transient and migratory (kinapses) contacts with antigen-presenting cells. Here, we describe how microchannels can be used to conveniently study the distinct dynamics of T cells during antigen recognition. Microchannels provide a controlled confined environment that promotes T cell migration and recapitulates kinapse and synapse behaviors when coated with appropriate pMHC molecules. We also depict the advantages of this in vitro approach for addressing mechanistic issues and for analysis.

https://www.ncbi.nlm.nih.gov/pubmed/28255711