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© Research
Publication : Journal of biotechnology

Lactate dehydrogenase (LDH) activity of the cultured eukaryotic cells as marker of the number of dead cells in the medium [corrected]

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of biotechnology - 01 Sep 1992

Legrand C, Bour JM, Jacob C, Capiaumont J, Martial A, Marc A, Wudtke M, Kretzmer G, Demangel C, Duval D

Link to Pubmed [PMID] – 1368802

J. Biotechnol. 1992 Sep;25(3):231-43

One significant problem in monitoring a culture’s evolution is to assess change in cell viability. We have demonstrated that LDH release could be a good indicator of cellular damage of many cell lines, especially during shear stress or sonication. Moreover, we have found a significant correlation between the number of dead cells, determined by Trypan Blue staining, and LDH activity measurements in the supernatant of hybridoma strains, whatever the culture conditions. We have also shown that when viability is still near 100% no LDH is released even at high cell concentrations. Therefore, LDH should serve as a potential marker of cell injury and death.

http://www.ncbi.nlm.nih.gov/pubmed/1368802