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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Intravital two-photon imaging of T-cell priming and tolerance in the lymph node

Team: Dynamics of Immune Responses
Member: Philippe Bousso
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2007

Celli S, Bousso P

Link to Pubmed [PMID] – 17876105

Methods Mol. Biol. 2007;380:355-63

Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.