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© Valérie Choumet
Mosquitoes were orally infected with the chikungunya virus. Midguts were dissected at day 5 post-infection, fixed and permeabilised. Virus is shown in red (anti-E2 protein, cyanine 3), the actin network in green (phalloidin 548) and nuclei in blue (DAPI).
Publication : Research in veterinary science

Interferon gamma response to Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigen L5P in cattle.

Team: Chemistry of Biomolecules
Team: UMR3523 – Chemistry for Life Sciences (Chem4Life)
Member: Christelle Ganneau
Member: Sylvie Bay
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Research in veterinary science - 01 Oct 2015

Holbert S, Branger M, Souriau A, Lamoureux B, Ganneau C, Richard G, Cochard T, Tholoniat C, Bay S, Winter N, Moyen JL, Biet F,

Link to Pubmed [PMID] – 26412530

Link to DOI – 10.1016/j.rvsc.2015.07.017S0034-5288(15)30025-4

Res Vet Sci 2015 Oct; 102(): 118-21

After Mycobacterium avium subsp. paratuberculosis (Map) infection the cell-mediated immune (CMI) response indicative of early Th1 activation may be detected using interferon-gamma release assay (IGRA). Currently, the purified protein derivatives (PPDs), i.e., the total extract of mycobacteria antigens are used to recall CMI responses against Map. This study aimed to assess the ability of the chemically synthesized Map specific cell wall lipopentapeptide L5P to induce CMI response in cows infected by Map compared to PPD. L5P and PPD elicited an IFN-γ response in 12 and 35 animals from two Map infected herds respectively, but IFN-γ was not detected in the 13 cows recruited from a non-infected herd. Levels of IFN-γ detected were higher with PPD than with L5P. There was no correlation between the IFN-γ response and the humoral response to Map or faecal culture.