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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Methods in molecular biology (Clifton, N.J.)

In situ fluorescence visualization of transcription sites and genomic Loci in blood stages of Plasmodium falciparum.

Team: Biology of Host-parasite Interactions
Team: Plasmodium Infection and Transmission
Member: Artur Scherf
Member: Liliana Mancio Silva
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2013

Mancio-Silva L, Scherf A,

Link to Pubmed [PMID] – 22990790

Methods Mol Biol 2013 ; 923(): 335-51

Fluorescence-based techniques have been used extensively in the malaria field to study the functional role of nuclear organization and gene positioning in blood stages of the human malaria parasite, Plasmodium falciparum. In this chapter, we present optimized protocols for bromouridine (BrUTP) incorporation into nascent RNA in live parasites and fluorescent in situ hybridization (FISH) in fixed parasites. Methodology to perform various combinations of the FISH assay, as well as a basic approach for quantitative analysis of nuclear position, is also described.