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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Methods in molecular biology (Clifton, N.J.)

Genome-wide chromatin immunoprecipitation-sequencing in Plasmodium

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2013

Lopez-Rubio JJ, Siegel TN, Scherf A

Link to Pubmed [PMID] – 22990789

Methods Mol. Biol. 2013;923:321-33

Chromatin immunoprecipitation (ChIP) studies have been used extensively in recent years to study the functional role of histone marks, variant histones, and other chromatin factors in gene expression in the human malaria parasite, Plasmodium falciparum. In this chapter, we present a ChIP-sequencing protocol optimized for blood-stage forms of this parasite. The processing of the immunoprecipitated DNA prior to high-throughput sequencing is performed in a way to minimize amplification biases due to the high genomic AT-content of the parasite.

http://www.ncbi.nlm.nih.gov/pubmed/22990789