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© Research
Publication : Frontiers in bioengineering and biotechnology

Imaging of Red-Shifted Light From Bioluminescent Tumors Using Fluorescence by Unbound Excitation From Luminescence

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Frontiers in bioengineering and biotechnology - 05 Apr 2019

Sônego F, Bouccara S, Pons T, Lequeux N, Danckaert A, Tinevez JY, Alam IS, Shorte SL, Tournebize R

Link to Pubmed [PMID] – 31024905

Front Bioeng Biotechnol 2019;7:73

Early detection of tumors is today a major challenge and requires sensitive imaging methodologies coupled with new efficient probes. optical bioluminescence imaging has been widely used in the field of preclinical oncology to visualize tumors and several cancer cell lines have been genetically modified to provide bioluminescence signals. However, the light emitted by the majority of commonly used luciferases is usually in the blue part of the visible spectrum, where tissue absorption is still very high, making deep tissue imaging non-optimal, and calling for optimized optical imaging methodologies. We have previously shown that red-shifting of bioluminescence signal by Fluorescence Unbound Excitation from Luminescence (FUEL) is a mean to increase bioluminescence signal sensitivity detection . Here, we applied FUEL to tumor detection in two different subcutaneous tumor models: the auto-luminescent human embryonic kidney (HEK293) cell line and the murine B16-F10 melanoma cell line previously transfected with a plasmid encoding the Luc2 firefly luciferase. Tumor size and bioluminescence were measured over time and tumor vascularization characterized. We then locally injected near infrared emitting Quantum Dots (NIR QDs) in the tumor site and observed a red-shifting of bioluminescence signal by (FUEL) indicating that FUEL could be used to allow deeper tumor detection in mice.

https://www.ncbi.nlm.nih.gov/pubmed/31024905