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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Identification of Lipoproteins Using Globomycin and Radioactive Palmitate

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2017

Buddelmeijer N

Link to Pubmed [PMID] – 28667603

Methods Mol. Biol. 2017;1615:75-80

Bacterial lipoproteins are characterized by fatty acids that are covalently attached to their amino terminus via posttranslational modification in the cytoplasmic membrane. Three enzymatic steps are involved in the synthesis of mature triacylated lipoprotein: prolipoprotein converts into diacylglyceryl-prolipoprotein that in turn converts into apolipoprotein, which is finally converted into mature triacylated lipoprotein. Here we describe the detection of one of these intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using H-palmitate labeling and inhibition by globomycin and detection by fluorography.

https://www.ncbi.nlm.nih.gov/pubmed/28667603