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© Melanie Blokesch, EPFL
Flagellated Vibrio cholerae
Publication : Nucleic acids research

Identification of key structural determinants of the IntI1 integron integrase that influence attC x attI1 recombination efficiency

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleic acids research - 20 Sep 2007

Demarre G, Frumerie C, Gopaul DN, Mazel D

Link to Pubmed [PMID] – 17884913

Nucleic Acids Res. 2007;35(19):6475-89

The integron platform codes for an integrase (IntI) from the tyrosine family of recombinases that mediates recombination between a proximal double-strand recombination site, attI and a single-strand target recombination site, attC. The attI site is only recognized by its cognate integrase, while the various tested attCs sites are recombined by several different IntI integrases. We have developed a genetic system to enrich and select mutants of IntI1 that provide a higher yield of recombination in order to identify key protein structural elements important for attC x attI1 recombination. We isolated mutants with higher activity on wild type and mutant attC sites. Interestingly, three out of four characterized IntI1 mutants selected on different substrates are mutants of the conserved aspartic acid in position 161. The IntI1 model we made based on the VchIntIA 3D structure suggests that substitution at this position, which plays a central role in multimer assembly, can increase or decrease the stability of the complex and accordingly influence the rate of attI x attC recombination versus attC x attC. These results suggest that there is a balance between the specificity of the protein and the protein/protein interactions in the recombination synapse.

http://www.ncbi.nlm.nih.gov/pubmed/17884913