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© MPI f. Dynamik und Selbstorganisation
Publication : European cytokine network

Human IL-Rbeta chains form IL-2 binding homodimers

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in European cytokine network - 26 Feb 2008

Pillet AH, Juffroy O, Mazard-Pasquier V, Moreau JL, Gesbert F, Chastagner P, Colle JH, Thèze J, Rose T

Link to Pubmed [PMID] – 18299274

Eur. Cytokine Netw. 2008 Mar;19(1):49-59

Two types of functional interleukin-2 receptor (IL-2Ralpha/IL-2Rbeta/gammac and IL-2Rbeta/gammac) have already been characterized in humans. Here we describe a new form consisting of IL-2Rbeta/beta homodimers that assemble spontaneously in the absence of gammac. Co-transfection of COS-7 cells with constructs expressing IL-2Rbeta chains tagged with either HA or MYC sequences results in the formation of IL-2Rbeta:HA/IL-2Rbeta:MYC complexes detectable by coimmunoprecipitation. The formation of these IL-2Rbeta:HA/IL-2Rbeta:MYC dimers is also observed in the absence of IL-2. Moreover, in COS cells expressing chimeras of IL-2Rbeta fused to fluorescence reporters such as IL-2Rbeta:ECFP and IL-2Rbeta:EYFP, we also observed specific FRET at the surface of living cells, as expected for dimer formation. Transiently transfected COS-7 cells expressing IL-2Rbeta bind 125I-labeled IL-2 (homodimers, Kd = 1nM) as cells expressing both IL-2Rbeta and gammac chains (heterodimers, Kd = 1 nM). IL-2Rbeta/IL-2Rbeta could represent either a decoy receptor or a new form of IL-2R involved in signaling when gammac expression is low.

http://www.ncbi.nlm.nih.gov/pubmed/18299274