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© Biologie structurale et chimie
Structure du domaine en doigt de zinc de la protéine NEMO, déterminée par Résonance magnétique nucléaire (RMN). Cette protéine jouant un rôle dans des maladies (cancer, inflammation), les connaissances acquises sur sa structure offrent de précieuses informations sur sa fonction.
Publication : Stem cell research

Generation of IPi002-A/B/C human induced pluripotent stem cell lines from MARCH amniotic fluid cells.

Team: Chemogenomic and Biological Screening Platform (PF-CCB)
Member: Mikaël Boullé
Member: Siham Schacre
Member: Alix Boucharlat
Member: Fabrice Agou
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Stem cell research - 19 Oct 2024

Boullé M, Leleu A, Schacre S, Banal C, Boucharlat A, Renault S, Hollenstein M, Frosk P, Yates F, Lefort N, Agou F

Link to Pubmed [PMID] – 39447316

Link to DOI – 10.1016/j.scr.2024.103589

Stem Cell Res 2024 Oct; 81(): 103589

Human induced pluripotent stem cells (hiPSCs) have become a revolutionary tool in biomedical research due to their unique in vitro properties and fate versatility. They offer insights into development or genetic disorders, facilitate drug discovery and hold promise for regenerative medicine. Here we generated three hiPSC cells – IPi002-A/B/C – from primary amniotic fluid cells (AFCs) obtained via amniocentesis for the prenatal diagnosis of MARCH syndrome: Multinucleated neurons, Anhydramnios, Renal dysplasia, Cerebellar hypoplasia, and Hydranencephaly. These AFCs underwent reprogramming through non-integrative viral transduction and the resulting hiPSCs exhibited normal karyotype and expressed typical pluripotency markers.