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© Biologie structurale et chimie
Structure du domaine en doigt de zinc de la protéine NEMO, déterminée par Résonance magnétique nucléaire (RMN). Cette protéine jouant un rôle dans des maladies (cancer, inflammation), les connaissances acquises sur sa structure offrent de précieuses informations sur sa fonction.
Publication : Stem cell research

Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells.

Team: Chemogenomic and Biological Screening Platform (PF-CCB)
Member: Mikaël Boullé
Member: Alix Boucharlat
Member: Fabrice Agou
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Stem cell research - 01 Apr 2024

Boullé M, Boucharlat A, Leleu A, Banal C, Coussement A, Hollenstein M, Yates F, Lefort N, Agou F

Link to Pubmed [PMID] – 38387169

Link to DOI – 10.1016/j.scr.2024.103350

Stem Cell Res 2024 Apr; 76(): 103350

Human induced Pluripotent Stem Cells (hiPSCs) represent an invaluable source of primary cells to investigate development, establish cell and disease models, provide material for regenerative medicine and allow more physiological high-content screenings. Here, we generated three healthy hiPSC control lines – IPi001-A/B/C – from primary amniotic fluid cells (AFCs), an infrequently used source of cells, which can be readily obtained from amniocentesis for the prenatal diagnosis of numerous genetic disorders. These AFCs were reprogrammed by non-integrative viral transduction. The resulting hiPSCs displayed normal karyotype and expressed classic pluripotency hallmarks.