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© Research
Publication : Journal of genetics and genomics = Yi chuan xue bao

Generation of a Double KO Mouse by Simultaneous Targeting of the Neighboring Genes Tmem176a and Tmem176b Using CRISPR/Cas9: Key Steps from Design to Genotyping

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of genetics and genomics = Yi chuan xue bao - 22 Apr 2016

Lemoine A, Chauveau-Le Friec G, Langa F, Louvet C

Link to Pubmed [PMID] – 27234594

J Genet Genomics 2016 05;43(5):329-40

The CRISPR/Cas9 system has been tailored to a revolutionary genetic tool because of its remarkable simplicity and efficacy. While complex genome editing in the mouse since the 1990s has been dominated by the use of embryonic stem (ES) cells, CRISPR/Cas9 now offers a versatile and fast approach to precisely modify virtually any DNA regions directly in mouse zygotes. Yet, this relative simplicity does not preclude a conscientious preparatory work that is often neglected when initiating a project. Here, we describe the key steps leading to successful generation of a double knockout (KO) mouse by simultaneously targeting two homolog genes, Tmem176a and Tmem176b, which are located in the same genomic locus. Additionally, we show that similar efficiency can be obtained in a mixed genetic background or directly in the C57BL/6 inbred strain. Thus, presented as a detailed case study that should be helpful to the non-specialists, we focus on the genotyping strategy to anticipate the various possibilities.

https://www.ncbi.nlm.nih.gov/pubmed/27234594