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© Research
Publication : Cellular microbiology

A New Nucleocytoplasmic RhoGAP Protein Contributes to Control the Pathogenicity of Entamoeba histolytica by Regulating EhRacC and EhRacD Activity

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Cellular microbiology - 23 Apr 2016

Hernandez-Flores A, de Jesus Almaraz-Barrera M, Lozano-Amado D, Correa-Basurto J, Rojo-Dominguez A, Luna-Rivera E, Schnoor M, Guillen N, Hernandez-Rivas R, Varga M

Link to Pubmed [PMID] – 27107405

Cell. Microbiol. 2016 Apr;

Small GTPases are signaling molecules that regulate important cellular processes. GTPases are deactivated by GTPase activating proteins (GAPs). While human GAPs have been intensively studied, no GAP has yet been characterized in Entamoeba histolytica. In this study, we identified and characterized a novel nucleocytoplasmic RhoGAP in E. histolytica termed EhRhoGAPnc. In silico analyses of the domain structure revealed a previously undescribed peptide region within the carboxy-terminal region of EhRhoGAPnc capable of interacting with phosphatidic acid and phosphatidylinositol 3,5-bisphosphate. The full structural GAP domain showed increase GAP activity compared to the minimum region able to display GAP activity, as analyzed both by experimental assays and molecular dynamics simulations. Furthermore, we identified amino acid residues that promote interactions between EhRhoGAPnc and its target GTPases EhRacC and EhRacD. Immunofluorescence studies revealed that EhRhoGAPnc colocalized with EhRacC and EhRacD during uroid formation but not during erythrophagocytosis. Interestingly, during erythrophagocytosis of red blood cells, EhRhoGAPnc colocalized with phosphatidic acid and phosphatidylinositol 3,5-bisphosphate. Overexpression of EhRhoGAPnc in E. histolytica led to inhibition of actin adhesion plate formation, migration, adhesion of E. histolytica to MDCK cells and consequently to an impairment of the cytopathic activity. This article is protected by copyright. All rights reserved.

http://www.ncbi.nlm.nih.gov/pubmed/27107405