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© Research
Publication : Current opinion in cell biology

FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Current opinion in cell biology - 21 Apr 2010

Mueller F, Mazza D, Stasevich TJ, McNally JG

Link to Pubmed [PMID] – 20413286

Curr. Opin. Cell Biol. 2010 Jun;22(3):403-11

The binding of nuclear proteins to chromatin in live cells has been analyzed by kinetic modeling procedures applied to experimental data from fluorescence recovery after photobleaching (FRAP). The kinetic models have yielded a number of important biological predictions about transcription, but concerns have arisen about the accuracy of these predictions. First, different studies using different kinetic models have arrived at very different predictions for the same or similar proteins. Second, some of these divergent predictions have been shown to arise from technical issues rather than biological differences. For confidence and accuracy, gold standards for the measurement of in vivo binding must be established by extensive cross validation using both different experimental methods and different kinetic modeling procedures.

http://www.ncbi.nlm.nih.gov/pubmed/20413286