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© Research
Publication : PLoS One

Fluid dynamics during bleb formation in migrating cells in vivo

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in PLoS One - 19 Feb 2019

Goudarzi M, Boquet-Pujadas A, Olivo-Marin JC, Raz E

Link to Pubmed [PMID] – 30807602

Link to DOI – 10.1371/journal.pone.0212699

PLoS One 2019

Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.