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© Research
Publication : Nature methods

Fast multicolor 3D imaging using aberration-corrected multifocus microscopy

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature methods - 09 Dec 2012

Abrahamsson S, Chen J, Hajj B, Stallinga S, Katsov AY, Wisniewski J, Mizuguchi G, Soule P, Mueller F, Dugast Darzacq C, Darzacq X, Wu C, Bargmann CI, Agard DA, Dahan M, Gustafsson MG

Link to Pubmed [PMID] – 23223154

Nat. Methods 2013 Jan;10(1):60-3

Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.

http://www.ncbi.nlm.nih.gov/pubmed/23223154