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© Research
Publication : STAR protocols

Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in STAR protocols - 18 Dec 2020

Ngondo RP, Cohen-Tannoudji M, Ciaudo C,

Link to Pubmed [PMID] – 33377021

Link to DOI – 10.1016/j.xpro.2020.100127

STAR Protoc 2020 Dec; 1(3): 100127

Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular markers to the definitive endoderm. Here, we present a fast procedure to identify a differentiation defect of mESCs toward ExEn in vitro through the molecular and cellular characterization of embryoid bodies, followed by direct differentiation of mESCs into ExEn. For complete details on the use and execution of this protocol, please refer to Ngondo et al. (2018).

https://pubmed.ncbi.nlm.nih.gov/33377021