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© Research
Publication : Molecular cell

Euryarchaeal genomes are folded into SMC-dependent loops and domains, but lack transcription-mediated compartmentalization.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular cell - 22 Dec 2020

Cockram C, Thierry A, Gorlas A, Lestini R, Koszul R,

Link to Pubmed [PMID] – 33382984

Link to DOI – S1097-2765(20)30905-910.1016/j.molcel.2020.12.013

Mol Cell 2021 02; 81(3): 459-472.e10

Hi-C has become a routine method for probing the 3D organization of genomes. However, when applied to prokaryotes and archaea, the current protocols are expensive and limited in their resolution. We develop a cost-effective Hi-C protocol to explore chromosome conformations of these two kingdoms at the gene or operon level. We first validate it on E. coli and V. cholera, generating sub-kilobase-resolution contact maps, and then apply it to the euryarchaeota H. volcanii, Hbt. salinarum, and T. kodakaraensis. With a resolution of up to 1 kb, we explore the diversity of chromosome folding in this phylum. In contrast to crenarchaeota, these euryarchaeota lack (active/inactive) compartment-like structures. Instead, their genomes are composed of self-interacting domains and chromatin loops. In H. volcanii, these structures are regulated by transcription and the archaeal structural maintenance of chromosomes (SMC) protein, further supporting the ubiquitous role of these processes in shaping the higher-order organization of genomes.

https://pubmed.ncbi.nlm.nih.gov/33382984